The PCR Song
There was a time when to amplify DNA
You had to grow tons and tons of tiny cells.
Then along came a guy named Dr. Kary Mullis,
Said you can amplify in vitro just as well.
Just mix your template with a buffer and some primers,
Nucleotides and polymerases too.
Denaturing, annealing and extending.
Well it's amazing what heating and cooling and heating will do.
PCR, when you need to detect mutations.
PCR, when you need to recombine.
PCR, when you need to find out who the daddy is.
PCR, when you need to solve a crime.
Practice of polymerase chain reaction (PCR) may require a license.
In my current job I use PCR mostly to make probes; in my old job I did a lot of QPCR to determine how many genetically engineered cells remained in a patient's blood. Once I thought it would be really cool to be one of those crime lab techs who uses PCR to solve crimes, but when I studied the job openings on the LAPD's website, it appeared that such techs have to gather their own samples-- that is, they have to go out to the crime scene, sometimes in the middle of the night, sometimes with the dead body right there, and try to collect DNA... yeah, I wasn't feeling that brave.